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ion xpresstm rna-seq barcode bc primer  (Thermo Fisher)


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    Thermo Fisher ion xpresstm rna-seq barcode bc primer
    Ion Xpresstm Rna Seq Barcode Bc Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ion+xpresstm+rna-seq+barcode+bc+primer/ion+xpresstm+rna+seq+barcode+bc+primer/pm37084144-69-25-39
    Average 90 stars, based on 1 article reviews
    ion xpresstm rna-seq barcode bc primer - by Bioz Stars, 2026-09
    90/100 stars

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    Purification:

    Article Title: miRNA Profiling of Developing Rat Retina in the First Three Postnatal Weeks.
    Article Snippet: For amplifying the cDNA, 3 μL of the purified cDNA was combined with 0.5 μL of Ion XpressTM RNA 3′ Barcode Primer, 0.5 μL of Ion XpressTM RNA-Seq Barcode BC primer (choose from BC01 to BC16 for different samples, ThermoFisher Scientific, MA, USA, Cat: 4475485), 22.5 1 3 μL of Platinum PCR SuperMix High Fidelity, reaction mix (26.5 μL) was then amplified using the following protocol: 94 °C for 2 min; (94 °C for 30 s, 50 °C for 30 s, and 68 °C for 30 s) for 2 cycles; (94 °C for 30 s, 62 °C for 30 s, and 68 °C for 30 s) for 16 cycles; 68 °C for 5 min.

    Polymerase Chain Reaction:

    Article Title: miRNA Profiling of Developing Rat Retina in the First Three Postnatal Weeks.
    Article Snippet: For amplifying the cDNA, 3 μL of the purified cDNA was combined with 0.5 μL of Ion XpressTM RNA 3′ Barcode Primer, 0.5 μL of Ion XpressTM RNA-Seq Barcode BC primer (choose from BC01 to BC16 for different samples, ThermoFisher Scientific, MA, USA, Cat: 4475485), 22.5 1 3 μL of Platinum PCR SuperMix High Fidelity, reaction mix (26.5 μL) was then amplified using the following protocol: 94 °C for 2 min; (94 °C for 30 s, 50 °C for 30 s, and 68 °C for 30 s) for 2 cycles; (94 °C for 30 s, 62 °C for 30 s, and 68 °C for 30 s) for 16 cycles; 68 °C for 5 min.

    Amplification:

    Article Title: miRNA Profiling of Developing Rat Retina in the First Three Postnatal Weeks.
    Article Snippet: For amplifying the cDNA, 3 μL of the purified cDNA was combined with 0.5 μL of Ion XpressTM RNA 3′ Barcode Primer, 0.5 μL of Ion XpressTM RNA-Seq Barcode BC primer (choose from BC01 to BC16 for different samples, ThermoFisher Scientific, MA, USA, Cat: 4475485), 22.5 1 3 μL of Platinum PCR SuperMix High Fidelity, reaction mix (26.5 μL) was then amplified using the following protocol: 94 °C for 2 min; (94 °C for 30 s, 50 °C for 30 s, and 68 °C for 30 s) for 2 cycles; (94 °C for 30 s, 62 °C for 30 s, and 68 °C for 30 s) for 16 cycles; 68 °C for 5 min.



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